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Goose desk top9/26/2023 The mischievous virtual goose is designed to playfully disrupt and annoy you while they work or browse your computer. The software was created by Samperson, and it gained popularity through viral videos. Inspired by the “Untitled Goose Game,” the Desktop Goose roams freely on your screen, creating chaos. If you would like to try other similar games, check out the free alternatives below. Desktop Goose is installed as a portable software, so this step will remove it completely from your computer. Navigate to the location where you saved the Desktop Goose Folder.If you no longer wish to have Desktop Goose on your computer, you can remove the application from your PC by following the instructions below: Have fun playing with the Goose! How to uninstall Desktop Goose from your PC Open the folder and run DesktopGoose.exe.įor Mac computers, simply extract the Goose folder to a preferred location on your computer and launch the program. If you’re using a PC, unpack the zip file into a new folder. ![]()
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Steps of western blotting technique9/26/2023 ![]() When an electric field is applied, the proteins move out of the polyacrylamide gel and onto the surface of the membrane, where the proteins become tightly attached. ![]() The process involves the use of porous pads and filter paper to facilitate the transfer. Electrophoretic transfer of proteins involves placing a protein-containing polyacrylamide gel in direct contact with a piece of nitrocellulose or other suitable, protein-binding support and "sandwiching" this between two electrodes submerged in a conducting solution. This method uses the electrophoretic mobility of proteins to transfer them from the gel to the membrane. The transfer method that is most commonly used for proteins is electroelution or electrophoretic transfer because of its speed and transfer efficiency. There are a variety of methods that have been used for this process that include, but are not limited to, diffusion transfer, capillary transfer, vacuum blotting transfer, and electroelution. Each system provides unique advantages when resolving proteins of different molecular weights.Ĭontinue reading: Overview of Protein ElectrophoresisĮxplore: Protein Gel electrophoresis productsįollowing electrophoresis, the protein must be transferred from the gel to a membrane. Several buffering systems or gel chemistries are available for protein gel electrophoresis. In contrast, sodium dodecyl sulfate-PAGE, or SDS-PAGE, separates proteins according to mass due to the negative charge imparted on proteins bound to the ionic SDS detergent. For example, nondenaturing PAGE, or native PAGE, separates proteins according to their mass-charge ratios. Several forms of PAGE exist and can offer different types of information about the protein(s) of interest. When combined with western blotting, PAGE is a powerful analytical tool providing information on the mass, charge, purity or presence of a protein. Proteins are commonly separated using polyacrylamide gel electrophoresis (PAGE) to characterize individual proteins in a complex sample or to examine multiple proteins within a single sample. Gel electrophoresis is a technique in which charged molecules, such as protein or DNA, are separated according to physical properties as they are forced through a gel by an electrical current. The indirect method offers many advantages over the direct method, which are described below. Labels (or conjugated molecules) may include biotin, fluorescent probes such as Invitrogen Alexa Flour or DyLight fluorophores, and enzyme conjugates such as horseradish peroxidase (HRP) or alkaline phosphatase (AP). Subsequently, the primary antibody is detected using an enzyme- or fluororophore-conjugated secondary antibody. In the indirect detection method, an unlabeled primary antibody is first used to bind to the antigen. This detection method is not widely used as most researchers prefer the indirect detection method for a variety of reasons. With the direct detection method, an enzyme- or fluorophore-conjugated primary antibody is used to detect the antigen of interest on the blot. One common variation involves direct versus indirect detection. Procedures vary widely for the detection step of a western blot experiment. Whatever system is used, the intensity of the signal should correlate with the abundance of the antigen on the membrane. Fluorescent blotting is a newer technique and is growing in popularity as it affords the potential to multiplex (detect multiple proteins on a single blot). Alternatively, fluorescently tagged antibodies can be used, which require detection using an instrument capable of capturing the fluorescent signal. However, digital imaging instruments based on charge-coupled device (CCD) cameras are becoming popular alternatives to film for capturing chemiluminescent signal. The light output can be captured using film. The most sensitive detection methods use a chemiluminescent substrate that produces light as a byproduct of the reaction with the enzyme conjugated to the antibody. ![]() Chromogenic substrates produce a precipitate on the membrane resulting in colorimetric changes visible to the eye. Often the secondary antibody is complexed with an enzyme, which when combined with an appropriate substrate, will produce a detectable signal. Most commonly, the transferred protein is then probed with a combination of antibodies: one antibody specific to the protein of interest (primary antibody) and another antibody specific to the host species of the primary antibody (secondary antibody). Next, the membrane is blocked to prevent any nonspecific binding of antibodies to the surface of the membrane. Subsequently, the separated molecules are transferred or blotted onto a second matrix, generally a nitrocellulose or polyvinylidene difluoride (PVDF) membrane. ![]() The first step in a western blotting procedure is to separate the macromolecules in a sample using gel electrophoresis.
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Git stash apply vs pop9/26/2023 ![]() The prerequisites for learning the git rebase command can be a basic understanding of Version Control Systems, Branching, and Git. On the other hand, the stash pop command in git removes or throws away the latest or the topmost stash. The git stash apply command leaves the topmost stash on the stash list so that we can later use it. Git stash apply is another command used for the same purpose. Before using the pop command, we should execute the git stash show -p command to display all the stashes. The git stash pop command enables the users to re-apply the commits using the git stash pop command only. The stash pop command will take the content from the stash file and then apply those changes to our current working file. It will delete all the stashes that exist in the repository.The git stash pop command helps us to remove or throw away the latest or the topmost stash. To delete all the available stashes, operate below command: $ git stash clear The stash clear command allows deleting all the available stashes at once. To delete a particular stash from the available stashes, pass the stash id in stash drop command. We can also delete a particular stash from the queue. The stash list command lists all the available stashes in the queue. In the above output, the most recent stash been dropped from given three stashes. The only way to revert it is if you do not close the terminal after deleting the stash. Caution should be taken before using stash drop command, as it is difficult to undo if once applied. Generally, it deletes the most recent stash. This command is used to delete a stash from the queue. The main difference between both of these commands is stash pop command that deletes the stash from the stack after it is applied. The stash pop command is quite similar to stash apply. The popping option removes the changes from stash and applies them to your working file. Git allows the user to re-apply the previous commits by using stash pop command. Git Stash Pop (Reapplying Stashed Changes) ![]() In case of more than one stash, you can use “stash apply” command followed by stash index id to apply the particular commit. It is showing output as “ Changes not staged for commit.” Consider the below output: $ git statusįrom the above output, you can see that the repository is restored to its previous state before stash. Now, if you will check the status of the repository, it will show the changes that are made on the file. The above output restores the last stash. To apply the commit, use the stash command, followed by the apply option. You can re-apply the changes that you just stashed by using the stash command. It will show all the stashes with indexing as so on. If we have more than one stash, then It will display all the stashes respectively with different stash id. To check the stored stashes, run the below command: $ git stash list ![]() The above stash will be saved with a message Git Stash List (Check the Stored Stashes) To stash a change with a message, run the below command: $ git stash save "" Git Stash Save (Saving Stashes with the message): At this point, you can switch between branches and work on them. My work is just stashed in its current position. We can check the status of the repository. ![]() To save it temporarily, we can use the stash command. To check the current status of the repository, run the git status command.įrom the above output, you can see the status that there are two untracked file Jenkinsfile and sts.yml available in the repository. To stash, let’s have a look at the repository’s current status. We can stash it to save as its current status. So I want to save it temporarily for future use. I am in a messy state, and I have not entirely edited any file yet. I have made changes to my project final-capstone in two files from two distinct branches. Let’s understand it with a real-time scenario. Some useful options are given below: Stashing Work Many options are available with git stash. Stashing takes the messy state of your working directory, and temporarily save it for further use. Generally, the stash’s meaning is “ store something safely in a hidden place.” The sense in git is also the same for stash Git temporarily saves your data safely without committing. The below figure demonstrates the properties and role of stashing concerning repository and working directory. The git stash command enables you to switch branches without committing the current branch. You don’t want to make a commit of half-done work. Sometimes you want to switch the branches, but you are working on an incomplete part of your current project.
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Macos ventura beta 99/25/2023 But with Ventura, it’s being upgraded to let you use your iPhone as a live camera in video calls. ![]() macOS Ventura review: Continuity Camera is handy in a pinchĪpple has offered a Continuity Camera feature since Mojave that lets you use an iPhone or iPad to snap photos and send them to your Mac. Stage Manager doesn’t seem to give you a lot of control over how tiles are ordered, so you can’t drag and drop the most important app in your workflow to the top of the list. Stage Manager can be customized, and at this stage in the beta process there are controls in the Desktop & Dock section of System Settings to control whether desktop icons and the tile pile on the left are hidden. And since the tiles show live previews of what’s going on in each app, I felt much more aware of what was going on in my Mail inbox, messaging apps and many browser windows whenever I glanced at the side of my screen. ![]() As much as it creates a bit of visual clutter, Stage Manager’s tile pile makes it easy to see at a glance what you have open. ![]() Once I switched it on (via a button in the Control Center), I found it a far better way of staying on top of what I'm working on than firing up Mission Control, or looking for telltale dots under icons on the Dock. Stage Manager is a simple tool for navigating and managing open applications, but since there are already multiple macOS tools for that (including Mission Control, Spaces and my personal favorite, the Cmd+Tab keyboard shortcut), I thought this new arrival would turn out to be as easily overlooked as the rest can be – I certainly used Spaces a few times, then never had cause to touch it again. Click on your Mac’s wallpaper and whatever is centered will be sent to the pile, allowing you an unimpeded view of your desktop, which can be otherwise hidden while Stage Manager is active. This lets you do things like nestle your Messages app alongside a Safari browser full of vacation locations you're currently discussing with friends in a group iMessage, allowing you to keep the conversation and your many plans neatly organized. If you drag one app into place over another that's currently centered in the screen, macOS Ventura will remember where you placed it and treat those two apps as one "tile" that you can select from the list. It’s a tool for managing apps and windows on your Mac desktop (learn how in our handy guide to how to use Stage Manager in macOS Ventura), and it works by centering whatever you’re currently focused on in the center of your display while displaying a stack of small tiles on the left-hand side of the screen that represent all the other apps you have open.Ĭlicking any Stage Manager tile will cause that app to move to center stage on your desktop, replacing whatever was there and moving it to the tile pile. Stage Manager is one of the new Ventura features I appreciate the most, though when it was first announced I thought it sounded superfluous. macOS Ventura review: Stage Manager is better than I expected To show you what I mean, let’s go over some of the biggest new features coming with macOS Ventura and how well they work in practice. And when Ventura’s new Freeform collaborative creation tool debuts later this year, having an iPhone or iPad handy make it much easier to diagram or doodle your ideas in shared workspaces. You'll now be able to hand off a FaceTime call (via Apple’s appropriately named new Handoff feature) from your iPhone/iPad to your Mac and vice versa. (Be sure to check out our guide on how to use your smartphone as a webcam.)įaceTime also gets an upgrade in macOS Ventura that will make it a bit more useful if you also own an iPhone. While propping up your iPhone near your Mac as an impromptu webcam feels a little silly, it’s a nice option to have should your Mac lack a webcam. ![]() We've spent some time testing out a prototype, and so far it works well - stay tuned for our full hands-on! (Image credit: Apple)įor example, the newly upgraded Continuity Camera feature lets you use an iPhone as a camera/microphone for your Mac. Apple's so confident in this "use-your-iPhone-like-a-webcam" Continuity Camera trick that it'll be selling special Belkin mounts for just that purpose later this year.
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Robin x chopper9/25/2023 ![]() ![]()
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Gta media player radio9/25/2023 ![]() You will now be able to listen to your own music by using the User Track Player. ![]() This is Radio Mirror Park from GTA V with old gen and new gen songs. Select the 'Scan User Tracks' button once: the game will start scanning your User Tracks folder. Back in 2014 I released this addon, during the Grand Theft Auto V craze, little did I know that itd become such an instant hit being one of my most subscribed and rated addons ever. This menu has three functions: Play Mode, Automatic Media Scan en Scan User Tracks. After adding the shortcut(s) you should start your game and go to Options > Audio Setup > User Tracks Options. The easiest way to do this is by creating one shortcut to your music folder, for example your music library. Like the weapon system, switching between radio stations is now done by bringing up a radio station wheel while in a vehicle to jump to any station, or turn off the radio. To play your own music you will have to create shortcuts to your music files in (My) Documents > GTA San Andreas User Files > User Tracks. As in previous Grand Theft Auto games, radio stations play a big part in Grand Theft Auto Online. The User Track Player allows you to skip a song or a radio commercial by pressing the key. It's quite easy to add your own music files to the User Track Player, since it doesn't require a lot of computer knowledge at all. Windows Media Player 9 and later versions have a wide range of codecs, including MP3 and WMA files. The player accepts 'Windows Wave' files (WAV) and 'Ogg Vorbis' files (OGG) by default, but the allowed formats can be extended by installing additional codecs. The User Track Player, which is only available in the PC version of GTA San Andreas, allows you to play your own music files. Radio: Creates a randomized playlist using audio files in the User Music folder, as well as inserting Rockstar-created station IDs, DJ banter and advertisements.īy default, you can use the - and = keys on the keyboard to cycle through songs, however this capability is disabled in Radio mode since it's simulating the playback of an on-the-air radio station.Main Page > Grand Theft Auto: San Andreas > Radio stations > User Track Player.Random: Creates a randomized playlist using the audio files in the User Music folder.Copy your compatible music files into the. Sequential: Plays the songs in the User Music folder in the order in which they appear. Gather your music files and then navigate to the GTA 5 Custom Music folder in C:\Users\ username\Documents\Rockstar Games\GTA V\User Music.There are three options for listening to your Self Radio station once you've successfully imported your music into GTA V: Description Starting from July 20, 2021, Media Sticks are made available for players to find in locations and inside players properties in Los Santos. ![]() Select Perform Quick Scan or Perform Full Scan for music.Open the Pause menu and go to the Settings tab.The game should automatically detect your audio files and add the Self Radiocomplete the following additional steps: Select Self Radio as your radio station.The question is pertinent since if you choose the media player radio to play, but the other players inside the car havent collected the pendrive yet, if they still hear the music. If you create a folder shortcut, all music files in that folder as well as any subfolders will automatically be included when scanned by GTA. Clearly he is talking about the new gta radio available called 'Media player', where you have to collect the 5 USB sticks to listen the musics.To create a shortcut, either hold Shift+Ctrl and then drag and drop files/folders, or select the files and use right-click Copy and then right-click Paste Shortcut.When gamers attempt to play the media player for the first time, without having. This saves on hard drive space by create small links to the files, rather than duplicating your music files. Although it is named media player, it is nothing but a unique radio station with unlockable tracks. Instead of directly copying music files, you can also create Shortcuts to music files or folders.Copy or move the desired audio files into the User Music folder. ![]() The media stick is located on one of the tables right near the whirlpool. So just land a helicopter on the building or use a lift to get to the roof. Open the Rockstar Games folder in My Documents ( > Users > My Documents > Rockstar Games ) USB-Stick 1: Blue EP You can find the media stick Blue EP on the roof of the Diamond Casino Resort in Los Santos.Users who play GTA 5 on PC can create their own radio stations, with track lists made up of their own music - or any other audio files you might choose to add.Īdding your own music to GTA 5 is easy, just follow these simple steps:
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![]() ![]() If you have any questions, please do not hesitate to ask them! Some vendors will be supporting releases for longer than six months. If you would like to download Java for free, you can get OpenJDK builds from the following vendors, among others: ![]() With the introduction of the new release cadence, many have asked where they should download Java, and if it is still free. Dev.java - Oracle's own Java learning platform.Hyperskill is a fairly new resource from Jetbrains (the maker of IntelliJ).MOOC Java Programming from the University of Helsinki.Google Drive, Dropbox, Mediafire, etc.- online IDE for many different programming languages.Codiva.io or Ideone for executable code snippets that use only the console.Github or Bitbucket repositories are also perfectly fine as are other dedicated source code hosting sites.Gist for multi-class programs, or programs that require additional files.Pastebin for programs that consist of a single class only.Copy your code into Redditlint, click Format + Copy, and paste the code in your post (remember to leave an empty line above the code!). Redditlint is a quick and simple code formatter for reddit code.For small bits of code (less than 50 lines in total, single classes only), the default code formatter is fine ( one blank line, then 4 spaces before each line).Do not submit executable jar or compressed (zip, rar, 7z, etc.) files!.No resource recommendations/promotions outside of the community resources thread Please post any recommendations and promotions of resources such as courses, websites and videos in the bi-weekly community resource thread.No piracy! We do neither tolerate requests for pirated material, nor do we allow advocating pirated material (even mentioning that you could download commercial products for free is forbidden) - such content will be removed without warning and the poster will automatically be permanently banned from the subreddit.PM help requests or offers will be removed without warning. Either ask your questions here and show your code, or you're out of luck. Comments with solutions will be removed and commenters will automatically be banned for a week. Do not ask for or reply with solutions as code, nor in plain text, rather comment explanations and guides.There might be other people with similar problems who could profit from the discussion in the thread. Do not delete your posts! Deleting is selfish and will deprive others of existing solutions.No Rewards: You may not ask for or offer payment when giving or receiving help.No links to your stackoverflow questions - we are not a second opinion to stackoverflow, nor are you going to get answers here when you didn't get satisfying ones there.No Processing Please use /r/processing instead.No MineCraft Please use /r/Minecraft instead. |